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Updated: Feb 10, 2026

Dural Stimulation and Periorbital von Frey Testing in Mice As a Preclinical Model of Headache
Published on: July 29, 2021
Rafael Gonzalez-Cano1, Bruno Boivin1, Daniel Bullock1
1Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA, United States.
This study introduces the Up-Down Reader (UDReader) software to automate the analysis of von Frey fiber tests for pain research. The tool accurately determines 50% pain thresholds from handwritten data, saving time and improving accuracy.
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