Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Predator-Prey Interactions02:39

Predator-Prey Interactions

21.7K
Predators consume prey for energy. Predators that acquire prey and prey that avoid predation both increase their chances of survival and reproduction (i.e., fitness). Routine predator-prey interactions elicit mutual adaptations that improve predator offenses, such as claws, teeth, and speed, as well as prey defenses, including crypsis, aposematism, and mimicry. Thus, predator-prey interactions resemble an evolutionary arms race.
21.7K
Implicit Differentiation01:25

Implicit Differentiation

69
In classical mechanics, motion is often described through relationships between spatial coordinates and time. A car moving along a straight highway with constant acceleration serves as a simple case where velocity is an explicit function of time. This scenario results in a linear equation, enabling straightforward analysis using basic differentiation techniques.In contrast, a satellite in circular orbit follows a path defined by an implicit function. The position of the satellite is constrained...
69
Logarithmic Differentiation01:28

Logarithmic Differentiation

75
When a car’s weight and driving forces act on a tire, they impose an external load on the rubber material. This load is resisted internally by forces distributed throughout the tire structure, which are defined as stress. The resulting deformation of the rubber due to this stress is quantified as strain. The relationship between stress and strain governs how the tire deforms under load and is central to understanding its mechanical response during operation.Rubber exhibits a nonlinear...
75
Cellular Differentiation00:57

Cellular Differentiation

5.5K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.5K
Differential Leveling01:12

Differential Leveling

731
Differential leveling is a precise method in surveying used to determine the elevation difference between two points. Its primary goal is to establish accurate vertical measurements to create level surfaces or grade lines critical for designing and constructing infrastructures such as roads, bridges, and buildings.The procedure for differential leveling begins with setting up and leveling the instrument at a point where the benchmark can be seen. The level rod is held on the benchmark (BM), and...
731
Integrator and Differentiator01:13

Integrator and Differentiator

1.5K
Op-amp circuits have significant applications in various fields, including automotive engineering. One such application is cruise control systems in cars, where op-amp circuits are integral for maintaining a constant speed. In these systems, op-amps function as both integrators and differentiators.
An integrator within an op-amp circuit produces an output directly proportional to the integral of the input signal. This is achieved by replacing the feedback resistor in a typical inverting...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mesenchymal Clock Regulates TMJ Homeostasis and Lipid Metabolic Rhythms with Age.

Journal of dental research·2025
Same author

miR-155-5p/Bmal1 Modulates the Senescence and Osteogenic Differentiation of Mouse BMSCs through the Hippo Signaling Pathway.

Stem cell reviews and reports·2023
Same author

The circadian clock has roles in mesenchymal stem cell fate decision.

Stem cell research & therapy·2022
Same author

Clock-modified mesenchymal stromal cells therapy rescues molecular circadian oscillation and age-related bone loss via miR142-3p/Bmal1/YAP signaling axis.

Cell death discovery·2022
Same author

Enhanced Circadian Clock in MSCs-Based Cytotherapy Ameliorates Age-Related Temporomandibular Joint Condyle Degeneration.

International journal of molecular sciences·2021
Same author

Correlation between gingival phenotype in the aesthetic zone and craniofacial profile-a CBCT-based study.

Clinical oral investigations·2020

Related Experiment Video

Updated: Feb 10, 2026

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
08:11

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Published on: January 31, 2019

11.8K

The Interaction between Bmal1 and Per2 in Mouse BMSC Osteogenic Differentiation.

Haiya Zhuo1, Yuhong Wang1, Qing Zhao1

  • 1State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Stem Cells International
|May 17, 2018
PubMed
Summary

The circadian clock

More Related Videos

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair
10:30

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Published on: May 23, 2014

11.1K
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

8.1K

Related Experiment Videos

Last Updated: Feb 10, 2026

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
08:11

Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Published on: January 31, 2019

11.8K
Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair
10:30

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Published on: May 23, 2014

11.1K
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

8.1K

Area of Science:

  • * Chronobiology and Stem Cell Biology
  • * Molecular mechanisms of circadian rhythm regulation in bone metabolism

Background:

  • * The circadian clock regulates physiological processes and behavior.
  • * BMAL1 (Brain and Muscle ARNT-like Protein 1) and Per2 (Period2) are key circadian clock components.
  • * Genetic deficiencies in Bmal1 or Per2 lead to altered bone volume.

Purpose of the Study:

  • * To investigate the role of BMAL1 and Per2 in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
  • * To determine if inhibiting BMAL1 and Per2 expression affects BMSC osteogenic differentiation.
  • * To assess the combined effect of inhibiting both BMAL1 and Per2 on BMSC osteogenesis.

Main Methods:

  • * Utilized lentiviral vectors with RNAi sequences to inhibit BMAL1 expression in BMSCs.
  • * Employed adenovirus vectors with RNAi sequences to suppress Per2 gene expression in BMSCs.
  • * Evaluated osteogenic differentiation capability following single and dual gene inhibition.

Main Results:

  • * Inhibition of BMAL1 expression in BMSCs enhanced osteogenic differentiation.
  • * Suppression of Per2 gene expression in BMSCs also increased osteogenic differentiation.
  • * Simultaneous inhibition of both BMAL1 and Per2 resulted in a more pronounced enhancement of osteogenic differentiation compared to single-gene inhibition.

Conclusions:

  • * BMAL1 and Per2 act as negative regulators of BMSC osteogenic differentiation.
  • * BMAL1 and Per2 exhibit a synergistic effect in suppressing BMSC osteogenic differentiation.
  • * Targeting BMAL1 and Per2 may offer therapeutic strategies for bone regeneration.