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Prevalence of bacteria and fungi in samples of cornea preservation fluid

Grażyna Broniek1, Ewa Langwińska-Wośko1,2, Małgorzata Sybilska1

  • 1SPKSO Ophthalmic University Hospital, Medical University of Warsaw, Warsaw, Poland.

Archives of Medical Science : AMS
|May 17, 2018
PubMed
Abstract

Insights

Corneal preservation fluid contamination with bacteria and fungi is a risk in transplants. However, no infections occurred in patients receiving contaminated corneas, suggesting prophylaxis effectiveness.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Infectious Diseases

Background:

  • Corneal transplant recipients face healthcare-associated infections from donor tissue.
  • Microbiological testing of cornea preservation fluid assesses infection risk.

Purpose of the Study:

  • To evaluate the risk of bacterial and fungal infections from cornea preservation fluid.
  • To analyze the susceptibility of isolates to gentamicin and patient outcomes.

Main Methods:

  • 725 cornea preservation fluid samples were cultured over 3 years (2011-2013).
  • Standard microbiological methods were used for identification and susceptibility testing.

Main Results:

  • 32 (4.4%) samples showed microbial growth (34 strains: 85.3% Gram-positive bacteria, 8.8% Gram-negative bacteria, 5.9% fungi).
  • 33.3% of bacterial isolates were resistant to gentamicin.
  • No infectious complications were observed in patients receiving corneas from contaminated fluid.

Conclusions:

  • Perioperative antibiotic prophylaxis is crucial for preventing donor cornea-derived infections.
  • Adding antifungal agents to cornea preservation fluids warrants consideration.
Keywords:
antibioticsbacteriacornea preservation mediacornea transplantationfungihealthcare-associated infections

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