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Arf1 and ER-mitochondrial tethering - a new trick for an old dog

Dana A Davis1

  • 1Department of Microbiology & Immunology, University of Minnesota, Minneapolis, MN, USA.

The FEBS Journal
|May 17, 2018
PubMed

Insights

The ERMES complex tethers the endoplasmic reticulum and mitochondria in fungi. Arf1 GTPase influences this tethering and impacts reactive oxygen species generation in Candida albicans.

Area of Science:

  • Cell biology
  • Mycology
  • Biochemistry

Background:

  • Endoplasmic reticulum (ER)-mitochondria tethering is vital for fungal mitochondrial functions.
  • The ER-mitochondria tethering (ERMES) complex mediates this interaction.
  • The GTPase Arf1, known for Golgi vesicular trafficking, also influences ER-mitochondria tethering.

Purpose of the Study:

  • To investigate the role of Arf1 in ER-mitochondria tethering via the ERMES complex in Candida albicans.
  • To understand how Arf1 impacts reactive oxygen species (ROS) generation in Candida albicans.

Main Methods:

  • Investigated Arf1's function in Candida albicans.
  • Assessed the impact of Arf1 on ERMES complex localization and function.
  • Measured reactive oxygen species generation in the presence and absence of Arf1 functional alterations.

Main Results:

  • Arf1 plays a role in ER-mitochondria tethering in Candida albicans, potentially through the ERMES complex.
  • Arf1 influences the generation of reactive oxygen species in this fungal species.
  • Arf1's functions extend beyond vesicular trafficking to include organelle tethering and immune defense molecule production.

Conclusions:

  • Arf1 is a multifunctional protein in Candida albicans, regulating vesicular trafficking, ER-mitochondria tethering, and reactive oxygen species production.
  • Understanding Arf1's diverse roles provides insights into fungal cell biology and host-pathogen interactions.

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