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Storage of spheroplasts at -70 degrees C for transfection with phi X174 RF DNA
H V Malling1, M C Fater, J G Burkhart
1National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Gene Analysis Techniques
|March 1, 1987
Abstract:
A method was developed for preparation of spheroplasts used for transfection with phi X174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70 degrees C.