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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

197.0K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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What is Gene Expression?01:36

What is Gene Expression?

11.6K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.9K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Related Experiment Video

Updated: Feb 10, 2026

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
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RNA-Seq and Expression Arrays: Selection Guidelines for Genome-Wide Expression Profiling.

Jessica Minnier1, Nathan D Pennock2, Qiuchen Guo2

  • 1School of Public Health, Oregon Health and Science University, Portland, OR, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 17, 2018
PubMed
Summary
This summary is machine-generated.

Explore gene expression using microarray and RNA-Seq technologies. This guide details their application, influencing factors, and provides recommendations for selecting the best platform for your research.

Keywords:
Differential expressionExpression arrayExpression profilingMassively parallel sequencingMicroarrayRNA-Seq

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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Gene expression profiling is crucial for understanding biological systems.
  • Microarray and massively parallel sequencing (RNA-Seq) are key technologies in this field.

Purpose of the Study:

  • To provide an overview of microarray and RNA-Seq for gene expression analysis.
  • To discuss factors affecting data generation and analysis for these platforms.
  • To offer recommendations for platform selection based on technical and analytical differences.

Main Methods:

  • Overview of microarray technology.
  • Overview of massively parallel sequencing (RNA-Seq) technology.
  • Comparative illustration study of protein-coding mRNA expression profiling.

Main Results:

  • Both platforms offer valuable gene expression profiling.
  • Technical and analytical differences impact performance.
  • An illustration study highlights similarities and differences.

Conclusions:

  • Platform selection depends on study-specific needs.
  • Consider technical and analytical aspects for optimal results.
  • Recommendations provided for informed platform choice.