Blood-direct InvaderPlus® as a new method for genetic testing
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Published on: December 14, 2006
Akira Hirasawa1, Tomoko Akahane2, Yusuke Tanigawara3
1Department of Obstetrics & Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. hir-aki@z8.keio.jp.
Aim:
This study compared the efficiency of Blood-direct InvaderPlus® with existing methods for detecting genetic polymorphisms by using clinical samples, and to determine whether this new method can be used for future clinical studies.
Materials & Methods:
We developed a new method, called Blood-direct InvaderPlus, for detecting genetic polymorphisms. Whole-blood samples were subjected to genetic analysis with the new method and also the conventional method.
Results:
The results obtained with the new method were consistent with those of direct sequencing. Blood-direct InvaderPlus was able to detect gene polymorphisms of UGT1A1*6, *27, *28, *60, VKORC1 -1639G>A, VKORC1 1173T>C and CYP2C9 *2 and *3 much faster than the conventional methods.
Conclusion:
Blood-direct InvaderPlus allowed accurate, simple and rapid detection of genetic polymorphisms.
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