Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

65.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.3K
PCR01:32

PCR

238.5K
Overview
238.5K
Nucleic Acids02:43

Nucleic Acids

50.7K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
50.7K
Nucleic acids02:43

Nucleic acids

190.6K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
190.6K
Nucleic Acids02:43

Nucleic Acids

9.0K
9.0K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.3K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibacterial Activities of Guava (Psidium guajava) Leaf Aqueous Extract Against Staphylococcus aureus and Escherichia coli.

Mymensingh medical journal : MMJ·2026
Same author

Preference and Actual Mode of Delivery, Complication and Outcome of Pregnancy among Women in a Tertiary Hospital of Bangladesh.

Mymensingh medical journal : MMJ·2026
Same author

Ambulatory Blood Pressure Monitoring Versus Casual Blood Pressure Measurement in Predicting Left Ventricular Hypertrophy in Children with Chronic Kidney Disease.

Mymensingh medical journal : MMJ·2025
Same author

Level of Depression, Anxiety and Stress among the Police Professionals in Bangladesh: A Nationwide Cross-sectional Study.

Mymensingh medical journal : MMJ·2025
Same author

Challenges and Opportunities of Management of Long Term Complications of Persons Affected By Leprosy in Context of Integrated Program in Bangladesh.

Mymensingh medical journal : MMJ·2025
Same author

Assessment of the Change of Blood pressure, Serum Total Cholesterol in Post Menopausal Women.

Mymensingh medical journal : MMJ·2025

Related Experiment Video

Updated: Feb 10, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

15.9K

Quantitative Analysis of Nucleic Acid Extraction Methods for Vibrio cholerae Using Real-time PCR and Conventional

Z Z Hossain1, J Ferdous, S M Tulsiani

  • 1Zenat Zebin Hossain, PhD Student, Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh.

Mymensingh Medical Journal : MMJ
|May 18, 2018
PubMed
Summary

The boiled template method is the most cost-effective and time-efficient DNA extraction technique for detecting Vibrio cholerae, especially in water samples, despite lower DNA yields compared to other methods.

More Related Videos

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

25.1K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.9K

Related Experiment Videos

Last Updated: Feb 10, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

15.9K
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

25.1K
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.9K

Area of Science:

  • Environmental Microbiology
  • Molecular Biology
  • Bacteriology

Background:

  • Cholera, a severe diarrheal disease, is caused by Vibrio cholerae.
  • Accurate detection of Vibrio cholerae relies on efficient nucleic acid extraction.
  • A rapid, cost-effective DNA extraction method is needed for diverse sample types.

Purpose of the Study:

  • To compare three DNA extraction methods for Vibrio cholerae detection.
  • To evaluate efficiency, speed, and cost-effectiveness.
  • To identify the optimal method for various sample matrices.

Main Methods:

  • Assessed boiled template, Phenol: Chloroform: Isoamyl alcohol, and QiaAmp® mini kit methods.
  • Tested crude culture, water, PBS, and food samples.
  • Utilized conventional and real-time PCR targeting the ctxA gene.

Main Results:

  • All methods yielded sufficient DNA for PCR detection.
  • The boiled template method showed lower DNA yield from water samples.
  • Boiled template method proved most suitable for resource-limited settings.

Conclusions:

  • The boiled template method is a practical and economical choice for Vibrio cholerae DNA extraction.
  • This method is particularly advantageous given time constraints and high sample volumes.
  • It enables reliable detection, especially in challenging environmental conditions.