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Related Experiment Video

Updated: Feb 10, 2026

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Published on: May 19, 2010

NDE1 positively regulates oligodendrocyte morphological differentiation.

Shoko Shimizu¹, Yugo Ishino², Masaya Tohyama^2,3

¹Division of Molecular Brain Science, Research Institute of Traditional Asian Medicine, Kindai University, Osaka-sayama, Osaka, 589-8511, Japan. shimizu@med.kindai.ac.jp.

Scientific Reports

|May 18, 2018

Summary

Nuclear Distribution E Homolog 1 (NDE1) is crucial for oligodendrocyte differentiation. NDE1 interaction with dynein is essential for myelin-forming cell process outgrowth and branching in the CNS.

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Last Updated: Feb 10, 2026

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

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Published on: May 19, 2010

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Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

Published on: August 21, 2011

Area of Science:

Neuroscience
Cell Biology
Developmental Biology

Background:

Oligodendrocytes form myelin sheaths around axons in the central nervous system (CNS).
Oligodendrocyte morphological differentiation, involving extensive process outgrowth, is vital for myelination.
The molecular mechanisms governing oligodendrocyte morphogenesis are not fully understood.

Purpose of the Study:

To investigate the role of Nuclear Distribution E Homolog 1 (NDE1) in oligodendrocyte morphological differentiation.
To elucidate the molecular mechanisms by which NDE1 influences oligodendrocyte development.

Main Methods:

Quantitative real-time PCR to detect Nde1 mRNA expression in mouse corpus callosum.
Small interfering RNA (siRNA) to knockdown NDE1 in primary oligodendrocytes.
Myelinating co-culture systems with dorsal root ganglion (DRG) neurons.
Immunoprecipitation assays to study protein interactions.
Overexpression of NDE1 variants in knockdown cells.

Main Results:

Nde1 mRNA is expressed in oligodendrocyte lineage cells during postnatal development.
NDE1 knockdown in vitro impairs oligodendrocyte process outgrowth, branching, and expression of myelin markers (CNPase, MBP).
NDE1 interacts with dynein intermediate chain (DIC) in oligodendrocytes.
NDE1 knockdown inhibits the ability of oligodendrocyte precursor cells (OPCs) to myelinate DRG axons.
Overexpression of a DIC-binding deficient NDE1 mutant mimics NDE1 knockdown effects.

Conclusions:

Nuclear Distribution E Homolog 1 (NDE1) is essential for oligodendrocyte morphological differentiation.
NDE1 regulates oligodendrocyte process formation and myelination through interaction with the dynein motor complex.
Understanding NDE1's role provides insights into CNS development and myelin disorders.