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Updated: Feb 10, 2026

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
Published on: October 8, 2018
Membrane association of monotopic phosphoglycosyl transferase underpins function
Leah C Ray1, Debasis Das2,3, Sonya Entova2
1Program in Biomolecular Pharmacology, Boston University School of Medicine, Boston, MA, USA.
Abstract:
Polyprenol phosphate phosphoglycosyl transferases (PGTs) catalyze the first membrane-committed step in assembly of essential glycoconjugates. Currently there is no structure-function information to describe how monotopic PGTs coordinate the reaction between membrane-embedded and soluble substrates. We describe the structure and mode of membrane association of PglC, a PGT from Campylobacter concisus. The structure reveals a unique architecture, provides mechanistic insight and identifies ligand-binding determinants for PglC and the monotopic PGT superfamily.
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