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Related Experiment Video

Updated: Feb 10, 2026

Phase Contrast and Differential Interference Contrast DIC Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

Isotropic differential phase contrast microscopy for quantitative phase bio-imaging.

Hsi-Hsun Chen¹, Yu-Zi Lin^2,3, Yuan Luo^3,4,5

¹Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan.

Journal of Biophotonics

|May 18, 2018

Summary

This summary is machine-generated.

We developed isotropic differential phase contrast (iDPC) for fast, accurate quantitative phase imaging. This method uses a programmable shield for structured illumination, enabling label-free live cell imaging.

Keywords:

biological imaging quantitative phase microscopy

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Area of Science:

Optical microscopy
Biomedical imaging
Quantitative Phase Imaging (QPI)

Background:

Quantitative phase imaging (QPI) retrieves optical phase information for biological and medical studies.
Differential phase contrast (DPC) microscopy recovers phase images using multi-axis intensity measurements.

Purpose of the Study:

To propose a novel method for isotropic differential phase contrast (iDPC) enabling high accuracy and stability.
To achieve phase recovery in a simple and high-speed manner for QPI.

Main Methods:

Implementation of iDPC using partially coherent microscopy.
Utilizing a programmable thin-film transistor (TFT) shield for digital modulation of structured illumination patterns.
Theoretical approach under partially coherent conditions.

Main Results:

Simulation results confirm the consistency of the iDPC theoretical approach under partial coherence.
Experimental demonstration of quantitative phase images for a micro-lens array.
Successful acquisition of label-free, live human cell images.

Conclusions:

The proposed iDPC method offers a simple, high-speed, and accurate approach for quantitative phase recovery.
iDPC is effective for imaging standard micro-lens arrays and label-free live biological samples.
This technique advances QPI applications in microscopy and medical studies.