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Updated: Feb 10, 2026
07:02
Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model
Published on: May 16, 2019
13.4K
Vitrification for bovine embryos with low-quality grade.
Hiroshi Tajimi1, Tsugumi Yamazaki2, Syoko Oike2
1Tajimi Bovine ET Clinic, Gifu, Japan.
Animal Science Journal = Nihon Chikusan Gakkaiho
|May 18, 2018
Summary
Vitrification using minimum volume cooling (MVC) and a simple one-step dilution method effectively preserves low-quality bovine embryos. This technique resulted in high survival rates and successful calf development, offering a practical solution for embryo preservation.
Area of Science:
- Reproductive Biology
- Embryology
- Cryobiology
Background:
- Vitrification is a cryopreservation technique.
- Preserving low-quality bovine embryos is challenging.
- Simple cryoprotectant dilution methods are needed for practitioners.
Purpose of the Study:
- To evaluate vitrification for low-quality bovine embryos.
- To assess a simple cryoprotectant dilution method.
- To optimize vitrification and warming protocols.
Main Methods:
- Minimum Volume Cooling (MVC) vitrification.
- One-step cryoprotectant dilution in straws.
- Post-warming embryo culture and assessment.
- In vivo development to term after vitrification.
Main Results:
- Vitrified embryos showed higher survival rates than frozen-thawed embryos.
- 100% survival was achieved with 0.3 mol/L sucrose warming.
- Vitrified-warmed embryos had comparable cell numbers to fresh controls.
- Six calves were born from 13 vitrified embryos.
Conclusions:
- MVC vitrification with one-step dilution is effective for low-quality bovine embryos.
- This method offers a practical approach for cryopreservation.
- Successful development to term demonstrates the technique's utility.
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