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Updated: Feb 10, 2026

Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
Comparative diagnostics of allergy using quantitative immuno-PCR and ELISA
Maria A Simonova1, Victor D Pivovarov1, Dmitry Y Ryazantsev1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 16/10, Miklukho-Maklaya St., 117997, Moscow, Russian Federation.
Aim:
Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens.
Results:
Single stranded 60-mer DNA conjugated to streptavidin was used to detect antigen-IgE-biotin complex by qiPCR. In semi-logarithmic scale qiPCR data were linear in a full range of serum dilutions resulting in three- to ten-times higher sensitivity of qiPCR in comparison with ELISA in IgE estimation in low titer sera.
Conclusion:
Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of qiPCR data.
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