Cytoplasmic membrane elaborations in oligodendrocytes during myelination of spinal motoneuron axons

The ultrastructure of paranodal oligodendroglial cytoplasm, which is located in proximity to the forming myelin sheath, was studied during maturation of spinal motoneuron axons in rat. At 8 days postnatal, the paranodal oligodendroglial loops contain a network of membrane-bound tubulovesicular elements. These membrane elaborations are most common in oligodendroglial loops attached to the outermost layers of the myelin sheath, i.e., paranodal loops closest to the nodal gap. The number of oligodendroglial cytoplasmic profiles per paranodal loop falls over the course of five to ten sequential paranodal loops, and these profiles are nearly absent in paranodal oligodendroglial cytoplasm located distant from the nodal gap. Oligodendrocytes in spinal cords of 14- and 20-day-old rats and of adult rats did not exhibit networks of tubulovesicular profiles. The appearance of these membrane organelles within oligodendroglial cytoplasm during myelin maturation suggests increased membrane turnover within paranodal cytoplasm located adjacent to the axon that is being myelinated. Membrane turnover within oligodendrocytes may reflect axonal modulation of glial function during myelination.