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Updated: Feb 10, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Separation of Four Flavonol Glycosides from Solanum rostratum Dunal Using Solvent Sublation Followed by HSCCC and Low
Qian Shao1, Lin Chang1, Zhenya Wei2
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
Abstract:
Hyperoside, 3'-O-methylquercetin 3-O-β-D-galactopyranoside, astragalin and 3'-O-methylquercetin 3-O-β-D-glucopyranoside from an invasive weed Solanum rostratum Dunal were separated and purified successfully by high-speed counter-current chromatography (HSCCC) with a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:7:1:7, v/v) and gradient elution mode preparative high-performance liquid chromatography (prep-HPLC) with low column temperature. In the sample pretreatment section, target compounds in aqueous extract of the weed were concentrated using solvent sublation. Two target fractions with purities of 93.75% and 93.68% were obtained from HSCCC. Their chemical structures were identified. The fraction 1 is a pure compound hyperoside and the fraction 2 is the mixture of astragalin, 3'-O-methylquercetin 3-O-β-D-galactopyranoside and 3'-O-methylquercetin 3-O-β-D-glucopyranoside by nuclear magnetic resonance and liquid chromatography-mass spectra. Then, the three flavonol glycosides in the fraction 2 were separated and purified successfully by prep-HPLC with low column temperature.
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