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Draft Genome Sequence of the Fish Pathogen Flavobacterium columnare Strain MS-FC-4
Ryan P Bartelme1, Paul Barbier2, Ryan S Lipscomb3
1School of Freshwater Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
Genome Announcements
|May 19, 2018
Summary
A highly virulent Flavobacterium columnare strain (MS-FC-4) from rainbow trout was sequenced. Its draft genome provides a foundation for understanding Flavobacterium columnare virulence and developing control strategies.
Area of Science:
- Aquatic microbiology
- Fish disease research
- Genomics
Background:
- Flavobacterium columnare is an important fish pathogen causing columnaris disease.
- Genetic group 1 strains, like MS-FC-4, are highly virulent.
- Rainbow trout (Oncorhynchus mykiss) are susceptible to F. columnare infections.
Purpose of the Study:
- To present the draft genome sequence of the highly virulent Flavobacterium columnare strain MS-FC-4.
- To provide a resource for functional genomic studies of F. columnare.
Main Methods:
- Isolation of Flavobacterium columnare MS-FC-4 from infected rainbow trout.
- Whole-genome sequencing and assembly of the F. columnare MS-FC-4 draft genome.
- Bioinformatic analysis to predict open reading frames (ORFs).
Main Results:
- The draft genome of F. columnare MS-FC-4 comprises three contigs.
- The total genome size is 3,449,277 base pairs.
- 2,811 open reading frames were predicted.
Conclusions:
- The sequenced draft genome of F. columnare MS-FC-4 is a valuable resource for future research.
- This genomic data will facilitate functional analyses to understand virulence mechanisms.
- The findings contribute to the development of strategies to combat Flavobacterium columnare infections in fish.
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