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Updated: Feb 10, 2026

Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays
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Dynamics of Aspen Roots Colonization by Pseudomonads Reveals Strain-Specific and Mycorrhizal-Specific Patterns of

Marie-Francoise Noirot-Gros1, Shalaka Shinde1, Peter E Larsen1

  • 1Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.

Frontiers in Microbiology
|May 19, 2018
PubMed

Insights

Plant growth promoting bacteria, Pseudomonas, form diverse root biofilms. Mycorrhization significantly alters these bacterial assemblies, influencing their structure and arrangement on plant roots.

Area of Science:

  • Microbiology
  • Plant Science
  • Ecology

Background:

  • Rhizosphere-associated Pseudomonas species are recognized as plant growth promoters (PGP) and mycorrhizal helper bacteria (MHB).
  • These bacteria influence plant health and ectomycorrhizal fungi interactions.
  • Understanding their colonization dynamics is crucial for agricultural and ecological applications.

Purpose of the Study:

  • To investigate the spatial and temporal dynamics of root colonization by specific Pseudomonas strains.
  • To examine how mycorrhization affects the root colonization patterns of these bacteria.
  • To model the observed bacterial assembly phenotypes.

Main Methods:

  • Utilized laboratory vertical plate systems for growing Aspen seedlings.
  • Inoculated seedlings with fluorescently labeled Pseudomonas fluorescens and Pseudomonas protegens strains.
  • Monitored root colonization over a 5-week period using microscopy.

Main Results:

  • Observed significant diversity in bacterial assemblies on seedling roots, which varied over time.
  • Mycorrhization strongly influenced bacterial assembly structure and arrangement.
  • Pseudomonas strains formed structured biofilms; on mycorrhizal roots, bacteria were embedded in mucilage in parallel arrangements.

Conclusions:

  • Bacterial assembly phenotypes are dynamic and influenced by root exudates and mycorrhizal status.
  • A model was developed to describe transitions between observed bacterial assembly phenotypes.
  • Root mucilaginous materials play a key role in driving bacterial assembly phenotypes.
Keywords:
Laccaria bicolorPopulus tremuloidesPseudomonas fluorescensbiofilmsmucilagemycorrhizationplant-root colonization

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