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Updated: Feb 10, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
Published on: May 8, 2015
Hierarchical shape-by-shape assembly of microparticles for micrometer-scale viral delivery of two different genes
Daewon Lee1, Amos Chungwon Lee1, Sangkwon Han2
1Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 08826, South Korea.
Abstract:
Understanding tissue engineering using a bottom-up approach has been hindered by technical limitations because no platform can demonstrate the controlled formation of a heterogeneous population of cells in microscale. Here, we demonstrate hierarchical shape-by-shape assembly of virus-laden particles into larger ones to transfect two different genes on the seeded cells. We show that smaller daughter particles with different sizes and shapes can be assembled into the matching indentations of larger parent particles with different sizes and shapes. Then, we transfected a population of cells with two different gene-transfecting viruses, each of which was laden on the parent or daughter particles.
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