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Updated: Feb 10, 2026

In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium
Published on: May 6, 2018
[Properties of decellularized rabbit carotid artery with two different cross-linked technologies: A comparative
Lei Huang1, Feng Kuang2, Demiao Kong1
1Department of Cardiovascular Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To compare the properties between decellularized rabbit carotid artery with different cross-linked technologies. Methods: The decellularized rabbit carotid arteries were randomly divided into a photo-oxidation group and a procyanidins group. One group was cross-linked with photo-oxidation and the other group was cross-linked with procyanidins. The in vitro or in vivo properties of the two groups were evaluated by testing heat-shrinking temperature, max tensile strength and the max elongation or by testing tissue structure, inflammatory reaction and calcification degree. Results: The heat-shrinking temperature, max tensile strength and the max elongation were similar in the two groups (P>0.05). The tissue structure and inflammatory reaction were also similar in the two groups. Although the result of Von-Kossa calcium salt stain was slightly different, the calcium content was lower in the procyanidins group than that in the photo-oxidation group (P<0.05). Conclusion: The grafts by two cross-linked technologies show excellent mechanical capability, lower immunogenicity, good biological stability and anti-calcification ability. The procyanidins group shows a better anti-calcification property than the photo-oxidation group.
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