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DNA Vector-based RNA Interference to Study Gene Function in Cancer
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DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

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[Effects of stromal interaction molecule 1 RNA interference on the cell proliferation and apoptosis in human

J Ye1, J Xie1

  • 1Department of Otolaryngology, Qinghai University Affiliated Hospital, Xining, 810001, China.

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi = Journal of Clinical Otorhinolaryngology Head and Neck Surgery
|May 19, 2018
PubMed

Insights

Stromal interaction molecule 1 (STIM1) RNA interference significantly reduced laryngeal cancer cell proliferation, invasion, and migration. STIM1 knockdown also increased apoptosis, offering potential therapeutic targets for Hep-2 cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Laryngeal cancer poses a significant health challenge.
  • Stromal interaction molecule 1 (STIM1) is implicated in various cellular processes.
  • Understanding STIM1's role in laryngeal cancer is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the impact of STIM1 RNA interference on human laryngeal cancer Hep-2 cells.
  • To assess the effects on cell proliferation, apoptosis, invasion, and migration in vitro.
  • To explore the molecular mechanisms underlying STIM1's function in laryngeal cancer.

Main Methods:

  • Lentivirus-mediated RNA interference was employed to silence STIM1 in Hep-2 cells.
  • Quantitative real-time PCR and Western blotting confirmed STIM1 knockdown.
  • Cell proliferation, invasion, migration, and apoptosis were analyzed using CCK-8, Transwell assays, scratch assays, and flow cytometry, respectively.

Main Results:

  • STIM1 gene and protein expression were significantly reduced post-interference.
  • Hep-2 cell proliferation, invasion, and migration abilities decreased (P<0.05).
  • Apoptosis rate increased, with upregulated caspase-3 and Bax, and downregulated Bcl-2, VEGF, and MMP9.

Conclusions:

  • STIM1 RNA interference effectively inhibits proliferation, invasion, and migration of human laryngeal cancer Hep-2 cells in vitro.
  • Silencing STIM1 enhances apoptosis in Hep-2 cells.
  • Targeting STIM1 presents a promising therapeutic strategy for laryngeal cancer.
Keywords:
STIM1 geneapoptosiscell proliferationhuman laryngeal cancerinvasionmigration

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