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Pdr18 is involved in yeast response to acetic acid stress counteracting the decrease of plasma membrane ergosterol
Cláudia P Godinho1, Catarina S Prata1, Sandra N Pinto1,2
1IBB - Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.
Scientific Reports
|May 20, 2018
Summary
This study reveals that Pdr18 protein helps yeast cells adapt to acetic acid stress by maintaining plasma membrane ergosterol levels. This adaptation is crucial for cellular homeostasis and resistance to toxic compounds.
Area of Science:
- * Molecular biology
- * Cellular stress response
- * Biochemistry
Background:
- * Saccharomyces cerevisiae exhibits resistance to various cytotoxic compounds through multistress resistance mechanisms.
- * Plasma membrane efflux pump proteins of the ABC superfamily are implicated in this resistance.
- * Pdr18 is a determinant of multistress resistance, potentially involved in ergosterol incorporation into the plasma membrane.
Purpose of the Study:
- * To investigate the biological role of Pdr18 in yeast's response to acetic acid stress.
- * To elucidate the impact of Pdr18 on plasma membrane properties under stress.
- * To confirm the involvement of Pdr18 in maintaining ergosterol homeostasis.
Main Methods:
- * Analysis of gene transcription during adaptation to acetic acid.
- * Monitoring of PDR18 and ergosterol biosynthetic genes (ERG2-4, ERG6, ERG24) expression.
- * Assessment of plasma membrane properties, including lipid order, permeability, and electrochemical potential.
Main Results:
- * Coordinated activation of PDR18 and ergosterol biosynthesis genes observed during adaptation to acetic acid.
- * Pdr18's role in maintaining plasma membrane ergosterol content in stressed cells was supported.
- * Pdr18 counteracts acetic acid-induced changes in plasma membrane lipid order, permeability, and electrochemical potential.
Conclusions:
- * Pdr18 is vital for yeast adaptation to acetic acid stress.
- * Pdr18-mediated resistance is intrinsically linked to ergosterol content and plasma membrane properties.
- * Maintaining plasma membrane integrity through ergosterol homeostasis is a key stress response mechanism.
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