Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
Self-Evaluation: Self-Enhancement and Self-Verification03:00

Self-Evaluation: Self-Enhancement and Self-Verification

5.8K
Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
5.8K
Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
What is Gene Expression?01:42

What is Gene Expression?

197.0K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
197.0K
Persuasion Strategies01:52

Persuasion Strategies

43.5K
Researchers have tested many persuasion strategies, including the foot-in-the door and the door-in-the-face techniques, in a variety of contexts. Ultimately, the principles are effective in selling products and changing people’s attitude, ideas, and behaviors (Cialdini & Goldstein, 2004).
43.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The dark genome in cardiovascular medicine.

European heart journal·2026
Same author

Bacterial infection reshapes monocyte and macrophage ontogeny at the CNS borders.

Science immunology·2026
Same author

Expression of GPR34 in microglia remains stable in human Alzheimer's disease.

Acta neuropathologica·2026
Same author

A deep-learning framework reveals whole-body perturbations at cell level.

Nature·2026
Same author

Widespread DNA off-targeting confounds RNA chromatin occupancy studies.

Nature biotechnology·2026
Same author

TMPRSS2-ERG confers resistance of prostate cancer to antiandrogens.

EMBO molecular medicine·2026

Related Experiment Video

Updated: Feb 10, 2026

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

11.6K

Re-evaluating microglia expression profiles using RiboTag and cell isolation strategies.

Zhana Haimon1, Alon Volaski1, Johannes Orthgiess2

  • 1Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.

Nature Immunology
|May 20, 2018
PubMed
Summary

RiboTag translatome profiling offers a more accurate view of microglia function than standard transcriptome sequencing. This method avoids artifacts from tissue dissociation, providing reliable data for brain research.

More Related Videos

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
09:34

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Published on: March 18, 2020

8.2K
Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
04:53

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Published on: April 5, 2022

4.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
10:00

RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

Published on: March 4, 2020

11.6K
Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection
09:34

Concomitant Isolation of Primary Astrocytes and Microglia for Protozoa Parasite Infection

Published on: March 18, 2020

8.2K
Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
04:53

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Published on: April 5, 2022

4.2K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genomics

Background:

  • Transcriptome profiling is crucial for understanding cell function and responses in physiology and pathophysiology.
  • Microglia, the brain's resident macrophages, are key players in neurological health and disease.

Purpose of the Study:

  • To compare the accuracy of standard cell-sorting-based transcriptome sequencing with the RiboTag method for profiling microglia.
  • To identify and address biases in conventional transcriptome profiling methods.

Main Methods:

  • Side-by-side comparison of classical cell-sorting transcriptome sequencing and RiboTag translatome sequencing in microglia.
  • Analysis of artifacts introduced by tissue dissociation, contamination, and non-ribosomal transcripts.

Main Results:

  • Conventional microglial transcriptomes contained significant artifacts from tissue dissociation and contamination.
  • RiboTag profiling provided a cleaner translatome, free from these artifacts.
  • RiboTag method proved valuable for assessing Cre recombinase expression accuracy in transgenic mice.

Conclusions:

  • Standard transcriptome profiling methods for microglia are prone to method-based biases and observer effects.
  • RiboTag translatome profiling is a valuable complementary strategy to standard methods.
  • This study establishes RiboTag as a reliable tool for accurate microglia research.