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Generation of genetically-engineered animals using engineered endonucleases.

Jong Geol Lee1,2, Young Hoon Sung3,4, In-Jeoung Baek5,6

  • 1ConveRgence mEDIcine research cenTer (CREDIT), Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea.

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|May 20, 2018
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Summary
This summary is machine-generated.

Engineered endonucleases enable precise genome editing in animals, creating better models for human diseases in drug discovery. These advanced tools accelerate the development of genetically engineered experimental animals for preclinical research.

Keywords:
CRISPREngineered endonucleaseGenome editingTALENZFN

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Area of Science:

  • Biotechnology
  • Genomics
  • Pharmacology

Background:

  • Successful drug discovery relies on accurate animal models for preclinical studies.
  • Engineered endonucleases offer precise genome editing capabilities.
  • These technologies are crucial for advancing experimental animal models.

Purpose of the Study:

  • To review recent advancements in engineered endonucleases for genome editing.
  • To discuss the application of these technologies in various laboratory animals.
  • To highlight the importance of genome editing in creating better animal models for human diseases.

Main Methods:

  • Utilizing engineered endonucleases such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR) systems.
  • Applying genome editing techniques to diverse laboratory animals including mice, rats, rabbits, dogs, pigs, and non-human primates.
  • Reviewing literature on the development and application of these technologies in preclinical research.

Main Results:

  • Engineered endonucleases are revolutionizing the generation of genetically engineered experimental animals.
  • These tools allow for efficient and precise genome editing, leading to improved animal models.
  • A wide range of species can now be modified to better mimic human diseases.

Conclusions:

  • Genome editing technologies are essential for developing more accurate animal models in drug discovery.
  • The application of engineered endonucleases significantly enhances preclinical research capabilities.
  • Further development in this field will improve the translation of research findings to human therapeutics.