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Updated: Feb 10, 2026

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
Published on: April 18, 2019
[Effect of bone morphogenetic protein-4 overexpression on the biological activity of mouse induced pluripotent stem
Jin Qiu1, Hui-Yu Zhang1, Li Liu2
1Dept. of Stomatology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Objective:
This study aimed to construct the expression of bone morphogenetic protein-4 (BMP4) lentiviral vector gene and explore its influence on the biological activity of mouse induced pluripotent stem (iPS) cells.
Methods:
iPS cell lines stably overexpressing BMP4 were constructed by lentivirus transfection (BMP4-overexpressing group). Cells without transfection served as the blank group, and cells with only vector transfection served as the empty-vector group. Cell proliferation was detected by CCK8, and the expression levels of ameloblastin (AMBN), cytokeratin (CK) 14, dentin sialophospho-protein (DSPP), bone sialoprotein (BSP), and Runx2 mRNA were detected by quantitative polymerase chain reaction. Alkaline phosphatase (ALP) activity was used to detect the degree of cell differentiation.
Results:
Compared with blank and empty-vector groups, proliferation activity and ALP activity of BMP4-overexpressing group obvious increased (P<0.05), BMP4, AMBN, CK14, DSPP, BSP, Runx2 mRNA expression also increased (P<0.05).
Conclusions:
BMP4 can significantly promote the odontogenic differentiation of iPS.
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