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Hollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells
Byung-Ju Jin1, Sujin Lee1, Alan S Verkman1
1Departments of Medicine and Physiology , University of California , San Francisco , California 94143-0521 , United States.
Analytical Chemistry
|May 22, 2018
Summary
A novel hollow micropillar array enables high-throughput compound screening using minimal patient-derived cells. This innovative technology facilitates drug discovery by efficiently testing thousands of compounds on cell cultures.
Area of Science:
- Biotechnology
- Cell Biology
- Drug Discovery
Background:
- High-throughput screening requires advanced assay formats for miniaturized screens.
- Patient-derived cells are valuable but limited in quantity for traditional assays.
Purpose of the Study:
- To develop a novel hollow micropillar array for compound screening.
- To enable high-capacity screening using small quantities of patient-derived cells.
Main Methods:
- Developed a hollow micropillar array for compound delivery in alginate hydrogel.
- Optimized array geometry and solution viscosity for sustained compound exposure with minimal cross-talk.
- Implemented the platform using CFTR-expressing epithelial cells and a fluorescent halide indicator.
Main Results:
- Successfully screened thousands of compounds using a single transwell filter with cultured cells.
- Demonstrated sustained compound exposure and minimal cross-talk between micropillar wells.
- Validated the platform for measuring CFTR chloride channel function.
Conclusions:
- The hollow micropillar array is a versatile platform for high-capacity drug screening.
- This method is applicable to various cell types cultured on solid or porous supports.
- Facilitates drug discovery with reduced cell usage and enhanced efficiency.
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