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Related Experiment Videos

Evolutionary conservation of laminin-binding proteins.

J D Lopes1, G F Da-Mota, C R Carneiro

  • 1Ludwig Institute for Cancer Research, São Paulo, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|January 1, 1988
PubMed
Summary

Pathogen virulence and cancer metastasis involve cell adhesion to laminin. This study reveals conserved laminin-binding sites across diverse species, suggesting a common evolutionary mechanism for cell adhesion and immune response enhancement.

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Area of Science:

  • Cell Biology
  • Immunology
  • Evolutionary Biology

Background:

  • Pathogen virulence and cancer metastasis correlate with cell adhesion to basement membrane components, particularly laminin.
  • Specific laminin receptors are crucial for this adhesion mechanism.
  • Laminin receptors have been identified in evolutionarily distant organisms, including human cells, parasites, and bacteria.

Purpose of the Study:

  • To investigate the role of laminin in the interaction between Staphylococcus aureus and macrophages.
  • To explore the evolutionary conservation of laminin-binding sites in different organisms.
  • To identify potential commonalities in laminin receptors across species.

Main Methods:

  • Investigated laminin's effect on Staphylococcus aureus phagocytosis by macrophages.

Related Experiment Videos

  • Utilized monoclonal antibodies (MAb) against bacterial laminin receptors to assess phagocytic enhancement.
  • Analyzed MAb binding to bacterial extracts and mammalian cell extracts to identify and characterize laminin-binding proteins.

    • Main Results:

    • Laminin enhances Staphylococcus aureus phagocytosis by macrophages in a species-specific manner.
    • Monoclonal antibody 1.H12, raised against the bacterial laminin receptor, inhibits this phagocytic enhancement.
    • MAb 1.H12 recognizes laminin-binding proteins in unicellular parasites and mammalian cells, eluting a 52-kDa protein from bacteria and a 67-kDa band from cancer cells.

    Conclusions:

    • The findings suggest an evolutionary conservation of the laminin-binding site across phylogenetically diverse organisms.
    • This conserved binding site likely plays a role in cell adhesion, immune responses, and potentially virulence and metastasis.
    • Monospecific monoclonal antibodies can identify conserved molecular structures involved in cell-matrix interactions.