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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

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Modified nucleoside triphosphates exist in mammals.

Han-Peng Jiang1, Jun Xiong1, Fei-Long Liu1

  • 1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) , Department of Chemistry , Wuhan University , Wuhan 430072 , P. R. China . Email: bfyuan@whu.edu.cn ; ; Tel: +86-27-68755595.

Chemical Science
|May 22, 2018
PubMed
Summary
This summary is machine-generated.

This study developed a new method to detect modified nucleoside triphosphates (NTPs) in cells. Many modified NTPs were found, with lower levels in cancer tissues, revealing their widespread existence in eukaryotes.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Nucleoside triphosphates (NTPs) undergo diverse chemical modifications impacting cellular processes.
  • Previous studies focused on enzyme-mediated modifications and in vitro incorporation of pre-modified NTPs.

Purpose of the Study:

  • To establish a sensitive method for determining endogenous modified NTPs in mammalian cells and tissues.
  • To investigate the presence and levels of modified NTPs in normal and cancerous tissues.

Main Methods:

  • Development of a chemical labeling method using 8-(diazomethyl)quinoline (8-DMQ).
  • Combination with liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) for sensitive detection.
  • Analysis of endogenous NTPs in mammalian cells and tissues.

Main Results:

  • Achieved 56-137 fold improvement in detection limits for NTPs.
  • Successfully determined 12 types of endogenous modified NTPs in mammalian cells and tissues.
  • Observed significantly decreased levels of most modified NTPs in human hepatocellular carcinoma (HCC) tissues compared to adjacent normal tissues.

Conclusions:

  • The study revealed the widespread existence of various modified NTPs in eukaryotes.
  • The developed LC-ESI-MS method provides a sensitive tool for analyzing endogenous modified NTPs.
  • Decreased modified NTP levels in HCC suggest potential roles in cancer development.