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A Millimeter Scale Flexural Testing System for Measuring the Mechanical Properties of Marine Sponge Spicules
Published on: October 11, 2017
Zhouia spongiae sp. nov., isolated from a marine sponge
Lingping Zhuang1,2, Binbin Lin1,2, Fei Qin1,2
1Xiamen Key Laboratory of Marine Medicinal Natural Products and Cell Engineering, Xiamen Medical College, Xiamen 361023, PR China.
Abstract:
A taxonomic study was carried out on strain HN-Y44T, which was isolated from sponge collected from Yangpu Bay, Hainan, China. Cells of strain HN-Y44T were Gram-stain-negative, non-motile, rod-shaped, yellow-pigmented and grew at 10-40 °C (optimum, 28 °C), at pH 6-10 (optimum, 7-8) and in 0-8 % (w/v) NaCl (optimum, 2-5 %). This isolate was positive for nitrate reduction, denitrification, oxidase, catalase and aesculin hydrolysis, but negative for indole production and urease. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HN-Y44T belongs to the genus Zhouia and is clearly distinct from the other described species of this genus, Zhouia amylolytica, with a 16S rRNA gene sequence similarity of 96.85 %. The dominant fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The major polar lipids comprised phosphatidylethanolamine, four unidentified aminolipids, four unidentified phospholipids and one unidentified lipid. The respiratory lipoquinone was identified as MK-6. The G+C content of the genomic DNA was 32.9 mol%. On the basis of the phenotypic and phylogenetic data, strain HN-Y44T represents a novel species of the genus Zhouia, for which the name Zhouia spongiae sp. nov. is proposed, with the type strain HN-Y44T (=MCCC 1K03329T=LMG 30460T).
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