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Feasibility of microRNA profiling in human inner ear perilymph

Matthew Shew1, Athanasia Warnecke2, Thomas Lenarz2

  • 1Departments of Otolaryngology Head and Neck Surgery.

Neuroreport
|May 22, 2018
PubMed

Insights

MicroRNAs (miRNAs) were successfully isolated from human perilymph, offering a potential new method for diagnosing inner ear disorders. These findings suggest miRNAs in perilymph could serve as biomarkers for hearing loss conditions.

Area of Science:

  • Otolaryngology
  • Molecular Biology
  • Genetics

Background:

  • Hearing loss stems from diverse molecular and cellular issues.
  • Current diagnosis relies on physiologic tests, patient history, and genetic testing, lacking direct inner ear tissue analysis.
  • MicroRNAs (miRNAs) are crucial regulators of cellular processes and emerging disease biomarkers.

Purpose of the Study:

  • To investigate the presence and potential of microRNAs (miRNAs) in human perilymph as biomarkers for inner ear diseases.
  • To establish the feasibility of collecting perilymph for miRNA analysis.

Main Methods:

  • Prospective collection of human perilymph samples from patients undergoing inner ear surgery.
  • Analysis of isolated miRNAs using Affymetrix GeneChip miRNA 4.0 microarrays.
  • Bioinformatic analysis of miRNA targets and associated pathways using Ingenuity Pathway Analysis.

Main Results:

  • MicroRNAs (miRNAs) were successfully isolated from all collected human perilymph samples.
  • Identified miRNAs are predicted to interact with genes expressed in the inner ear.
  • Perilymph miRNAs correlate with pathways implicated in hearing disorders.

Conclusions:

  • Human perilymph sampling is a feasible method for miRNA isolation.
  • Perilymph miRNAs show promise as potential biomarkers for diagnosing inner ear disorders and hearing loss.
  • This approach could lead to novel diagnostic tools for inner ear conditions.

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