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Related Concept Videos

Group Polarization01:01

Group Polarization

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Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.
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Mass Spectrometry: Overview01:19

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry of Amines01:15

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In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic...
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Mass Spectrometry: Isotope Effect01:13

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Related Experiment Video

Updated: Feb 10, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Mapping Cellular Polarity Networks Using Mass Spectrometry-based Strategies.

Avais M Daulat1, Tania M Puvirajesinghe1, Luc Camoin2

  • 1Centre de Recherche en Cancérologie de Marseille (CRCM), 'Cell Polarity, Cell Signalling, and Cancer', Equipe Labellisée Ligue Contre le Cancer, Aix Marseille Univ, CNRS, INSERM, Institut Paoli-Calmettes, CRCM, Marseille, France.

Journal of Molecular Biology
|May 22, 2018
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Summary

Cell polarity is crucial for tissue function and development. Proteomics, particularly mass spectrometry, is key to understanding the complex protein networks governing cell polarity and its role in diseases.

Keywords:
epithelial polaritymass spectrometrypolarity proteinsprotein complexproteomics

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell polarity is essential for tissue homeostasis, embryonic development, and overall health.
  • Defects in cell polarity are linked to various diseases.
  • Understanding the molecular basis of cell polarity networks is crucial but complex.

Purpose of the Study:

  • To review the application of mass spectrometry in studying cell polarity.
  • To elucidate the spatio-temporal organization and regulation of cell polarity protein networks.
  • To highlight the role of cell polarity in normal and pathological contexts, focusing on epithelial tissues.

Main Methods:

  • Review of mass spectrometry techniques for proteomics.
  • Analysis of how mass spectrometry identifies protein complexes and modifications.
  • Integration of mass spectrometry with molecular strategies for in situ proteomic analysis.

Main Results:

  • Mass spectrometry has been instrumental in dissecting cell polarity networks at tissue and cellular levels.
  • Detailed insights into interconnections between polarity proteins and signaling pathways have been gained.
  • The dynamic nature of cell polarity networks is being revealed through proteomic approaches.

Conclusions:

  • Proteomics, especially mass spectrometry, offers powerful tools to unravel cell polarity mechanisms.
  • This approach provides a comprehensive view of protein networks in normal physiology and disease.
  • Future advancements in mass spectrometry promise further breakthroughs in understanding cell polarity.