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Updated: Feb 10, 2026

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
Published on: March 17, 2023
Characterization and coagulation-flocculation performance of a composite flocculant in high-turbidity drinking water
Xiang Xia1, Shuhuan Lan2, Xudong Li1
1Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, PR China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, PR China; University of Chinese Academy of Sciences, 100049, Beijing, PR China.
Abstract:
Klebsiella variicola B16, a microbial bioflocculant (MBF-B16)-producing bacteria, was isolated and identified by its 16S rRNA sequence, biochemical properties, and physiological characteristics. The effects of culture conditions on MBF-B16 production, including carbon source, nitrogen source, C/N ratio, initial pH, and culture temperature, were investigated in this study. Results showed that 6.96 g of MBF-B16 could be extracted from a 1-L culture broth under optimized conditions. Chemical analysis showed that polysaccharide and protein were the main components. The neutral sugar consisted of galactose only, which was proposed in Klebsiella genus for the first time. In addition, a composite flocculant (CF) that contains polyaluminum ferric chloride (PAFC) and MBF-B16 for the removal of turbidity and SS in drinking water was optimized by response surface methodology. CF could reduce PAFC dosage by about 56.2-72%. Charge neutralization and adsorption bridging effect were the primary flocculation mechanisms.
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