Molecular Analysis of a bla_IMP-1-Harboring Class 3 Integron in Multidrug-Resistant Pseudomonas fulva

Area of Science:

• Microbiology
• Genetics
• Antimicrobial Resistance

Background:

• A multidrug-resistant (MDR) Pseudomonas fulva strain was isolated from a urine sample.
• The study focused on understanding the genetic basis of MDR in this bacterial isolate.

Purpose of the Study:

• To characterize the novel class 3 integron carrying the blaIMP-1 gene.
• To investigate mutations in quinolone-resistance-determining regions (QRDRs) contributing to MDR.

Main Methods:

• Isolation and identification of Pseudomonas fulva.
• Molecular analysis including gene sequencing to identify blaIMP-1 and mutations in gyrA and parC.

Main Results:

• The isolate harbored a novel, full-length, chromosomal Tn402-like class 3 integron containing the gene cassette array aacA31-fosE-blaIMP-1.
• Two mutations in gyrA and one mutation in parC were identified within the QRDRs.

Conclusions:

• The novel blaIMP-1-carrying class 3 integron is reported.
• The identified integron and QRDR mutations likely contributed to the observed multidrug-resistant phenotype in Pseudomonas fulva.