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Updated: Feb 10, 2026

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
Published on: September 16, 2013
An Oil-Free Picodrop Bioassay Platform for Synthetic Biology
Christian A Siltanen1, Russell H Cole1, Sean Poust1
1Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Abstract:
Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation.
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