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Updated: Feb 10, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO
Marie-Alice Fraiture1,2, Assia Saltykova1,3, Stefan Hoffman1
1Scientific Institute of Public Health (WIV-ISP), Platform of Biotechnology and Bioinformatics (PBB), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Abstract:
In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette. In the present study, we investigate the feasibility to integrate the described workflow with the MinION Next-Generation-Sequencing (NGS). The MinION sequencing platform can provide long read-lengths and deal with heterogenic DNA libraries, allowing for rapid and efficient delivery of sequences of interest. In addition, the ability of this NGS platform to characterize unauthorized and unknown GMO without any a priori knowledge has been assessed.
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