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Circular RNA 0068669 as a new biomarker for hepatocellular carcinoma metastasis

Ting Yao1, Qingqing Chen1, Zhouwei Shao1

  • 1Department of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.

Journal of Clinical Laboratory Analysis
|May 23, 2018
PubMed

Insights

Circular RNAs (circRNAs) are implicated in disease. This study found hsa_circ_0068669 is downregulated in hepatocellular carcinoma (HCC), suggesting its potential as a biomarker for HCC metastasis.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in disease pathogenesis.
  • The diagnostic potential of circRNAs in hepatocellular carcinoma (HCC) remains largely unexplored.
  • This study focuses on hsa_circ_0068669, a circRNA associated with HCC.

Purpose of the Study:

  • To investigate the diagnostic value of hsa_circ_0068669 in hepatocellular carcinoma (HCC).
  • To evaluate hsa_circ_0068669 as a potential biomarker for HCC diagnosis and metastasis.

Main Methods:

  • Real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to measure hsa_circ_0068669 expression levels.
  • Expression analysis was conducted in HCC tissues, adjacent non-tumorous tissues, HCC cell lines, and chronic hepatitis tissues.
  • Statistical analyses, including t-tests, ANOVA, and ROC curve analysis, were employed to assess diagnostic value and correlations.

Main Results:

  • Hsa_circ_0068669 expression was significantly downregulated in HCC tissues and cell lines compared to normal controls.
  • Expression levels were also decreased in HCC tissues relative to chronic hepatitis tissues.
  • Downregulation of hsa_circ_0068669 correlated with microvascular invasion and TNM staging in HCC patients.

Conclusions:

  • Hsa_circ_0068669 exhibits significantly reduced expression in hepatocellular carcinoma.
  • The findings suggest hsa_circ_0068669 could serve as a novel biomarker for HCC metastasis.
Abstract

Keywords:
biomarkercircular RNAgene expressionhepatocellular carcinomametastasis

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