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Cardiac Hypertrophy is Positively Regulated by MicroRNA‑24 in Rats

Juan Gao1, Min Zhu1, Rui-Feng Liu1

  • 1Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital; Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education; Beijing Key Laboratory of Cardiovascular Receptors Research, Beijing 100191, China.

Chinese Medical Journal
|May 23, 2018
PubMed
Abstract

Insights

MicroRNA-24 (miR-24) promotes cardiac hypertrophy by down-regulating p27 expression, impacting the cell cycle. This study investigates miR-24

Area of Science:

  • Cardiovascular Biology
  • Molecular Cardiology
  • MicroRNA Research

Background:

  • MicroRNA-24 (miR-24) is implicated in heart failure by affecting myocardial excitation-contraction coupling.
  • The role of miR-24 in cardiac hypertrophy, a precursor to heart failure, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of miR-24 in cardiac hypertrophy.
  • To elucidate the underlying mechanisms of miR-24's action in cardiac hypertrophy.

Main Methods:

  • Utilized a rat model of transverse aortic constriction (TAC) to induce cardiac hypertrophy.
  • Employed echocardiography, histology, real-time PCR, luciferase assays, immunofluorescence, and 3H-leucine incorporation.
  • Analyzed cell cycle progression using flow cytometry in neonatal rat cardiac myocytes (NRCMs).

Main Results:

  • miR-24 expression was significantly increased in TAC-induced cardiac hypertrophy.
  • CDKN1B (p27, Kip1), a cell cycle regulator, was identified as a direct target of miR-24.
  • Overexpression of miR-24 in NRCMs reduced p27 levels, inhibited G0/G1 cell cycle arrest, and promoted cardiomyocyte hypertrophy.

Conclusions:

  • miR-24 contributes to cardiac hypertrophy.
  • This effect is partly mediated by the down-regulation of p27 expression, influencing the cell cycle.
  • miR-24 represents a potential therapeutic target for cardiac hypertrophy.
Keywords:
Cardiac HypertrophyCell CycleMicroRNAMicroRNA-24p27

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