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Updated: Feb 10, 2026

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
Published on: July 29, 2018
Reversal of siRNA-mediated gene silencing in vivo
Ivan Zlatev1, Adam Castoreno1, Christopher R Brown1
1Alnylam Pharmaceuticals, Cambridge, Massachusetts, USA.
Abstract:
We report rapid, potent reversal of GalNAc-siRNA-mediated RNA interference (RNAi) activity in vivo with short, synthetic, high-affinity oligonucleotides complementary to the siRNA guide strand. We found that 9-mers with five locked nucleic acids (LNAs) have the highest potency across several targets. Our modular, sequence-specific approach, named REVERSIR, may enhance the therapeutic profile of any long-acting GalNAc-siRNA (short interfering RNA) conjugate by enabling control of RNAi pharmacology.
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