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Updated: Feb 10, 2026
08:02
Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
Published on: January 7, 2019
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[EVALUATION OF AN OPTIMIZING PROTOCOL FOR FABRICATING A SCAFFOLD DERIVED FROM PORCINE SKELETAL MUSCLE EXTRACELLULAR
Di Zhang1,2, Qiuwen Tan1,2, Shenli Tang1,2
1Center of Breast Surgery, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P. R. China.
Summary
This study presents an optimized decellularization protocol for porcine skeletal muscle tissue, creating a cell-free matrix (DPMT) with preserved collagen. The DPMT scaffold demonstrates excellent biocompatibility and promotes neovascularization in vivo.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Context:
- Developing ideal scaffolds is crucial for tissue regeneration.
- Porcine skeletal muscle acellular matrix (DPMT) offers potential for tissue engineering applications.
- Optimizing decellularization protocols is key to preserving scaffold integrity and biological function.
Purpose:
- To explore an optimized protocol for decellularizing porcine skeletal muscle tissue.
- To fabricate an ideal scaffold from porcine skeletal muscle acellular matrix (DPMT).
- To evaluate the biocompatibility and degradation profile of the DPMT scaffold in vitro and in vivo.
Summary:
- A serial-step homogenating-milling-detergent method effectively decellularized porcine skeletal muscle within one day.
- The resulting DPMT scaffold was cell and lipid-free, retaining collagen type I and IV, with significantly reduced DNA and protein content compared to native tissue.
- In vitro and in vivo studies demonstrated good cytocompatibility, minimal inflammatory response after initial implantation, and promoted neovascularization.
Impact:
- The developed protocol is effective, time-saving, and reproducible for creating DPMT scaffolds.
- DPMT exhibits good biocompatibility and potential for promoting neovascularization, making it a promising material for tissue engineering.
- This research contributes to the development of advanced biomaterials for regenerative medicine applications.
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