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Updated: Feb 10, 2026
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
[Effects of over-expressing smad7 gene on keloid fibroblasts]
Zhenwei Chen1, Yin Liu1, Minqin Xiao1
1Department of Plastic Surgery, Second Affiliated Hospital, Kunming Medical University, Kunming Yunnan, 650101, P. R. China.
Objective:
?To explore if Smad7 protein can inhibit growth of keloids by observing the gene and protein expressions of Smad7, collagen type I, and collagen type Ⅲ and cell proliferation after over-expression vectors of Smad7 transfecting keloid fibroblasts (KFb).
Methods:
?Fibroblasts were acquired from 10 male patient with keloids at the age of 20 to 25 years. After in vitro culture, KFb were divided into 3 groups: untransfected group (group A), pcDNA3.1 (-) transfected group (group B), and pcDNA3.1 (-) -smad7 transfected group (group C). The mRNA and protein expression levels of Smad7, collagen type I, and collagen type Ⅲ were detected by real-time fluorescence quantitative PCR and Western blot at 48 hours after transfection. The cell proliferation ability was detected by MTT assay at 24 hours after transfection.
Results:
?The relative expression levels of mRNA and protein of Smad7 in group C were significantly higher than those in group A and group B (P<0.01). The relative expression levels of mRNA and protein of collagen type I and collagen type Ⅲ in group C were significantly lower than those in group A and group B (P<0.01). The relative expression levels of mRNA of collagen type I and collagen type Ⅲ in group B were significantly higher than those in group A (P<0.01); and the relative expression levels of proteins of Smad7, collagen type I, and collagen type Ⅲ were significantly lower than those in group A (P<0.01). The cell proliferation ability in group C was significantly lower than that in group A and group B at each time point by MTT assay (P<0.05), but no difference was found between group A and group B (P>0.05).
Conclusions:
?Gene expressions of collagen type I, and collagen type Ⅲ and cell proliferation will be inhibited after KFb are transfected by over-expression vector of Smad7.
Insights
Smad7 protein overexpression significantly inhibited keloid fibroblast proliferation and reduced collagen production. This suggests Smad7 is a potential therapeutic target for keloid treatment.
Area of Science:
- Cell Biology
- Dermatology
- Molecular Biology
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