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Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
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Functional Studies on Steroid Receptors

Simon Schlanger1, Hannelore V Heemers2,3,4

  • 1Department of Cancer Biology, Cleveland Clinic, Lerner Research Institute, Cleveland, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 23, 2018
PubMed

Insights

Prostate cancer progression involves altered androgen receptor (AR) coregulators. Modulating these AR-associated proteins with small molecules offers a potential therapeutic strategy for prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Nuclear receptors are crucial in prostate cancer.
  • The androgen receptor (AR) is a key transcription factor regulating cellular events.
  • AR-associated coregulators can be up- or down-regulated in prostate cancer.

Purpose of the Study:

  • To investigate the role of AR-associated coregulators in prostate cancer.
  • To explore the potential of modulating AR-associated proteins for cancer therapy.

Main Methods:

  • Gene silencing and overexpression techniques.
  • Hormonal treatment.
  • Real-time RT-PCR and Chromatin Immunoprecipitation (ChIP) assays.

Main Results:

  • Altered expression of AR-associated regulators may enhance androgen-induced proliferation, migration, and invasion.
  • Investigated expression and function of AR-associated proteins.

Conclusions:

  • AR-associated coregulators are implicated in prostate cancer development.
  • Targeting AR-associated proteins with small molecule inhibitors presents a promising therapeutic avenue for prostate cancer.
Keywords:
Androgen receptorCoregulatory proteinsProstate cancer progressionTranscriptional complexes

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