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Related Experiment Video

Updated: Feb 10, 2026

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Published on: June 13, 2019

Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer.

Chris W D Armstrong¹, Oksana Lyubomska¹, Melissa J LaBonte¹

¹Centre for Cancer Research and Cell Biology (CCRCB), Queen's University Belfast, Belfast, UK.

Methods in Molecular Biology (Clifton, N.J.)

|May 23, 2018

Summary

Genetically engineered mouse models (GEMMs) are crucial for cancer research, but generating age-matched cohorts is challenging. Deriving cell lines from GEMMs overcomes this limitation, enabling syngeneic models for immunocompetent studies.

Keywords:

Cell line derivation Cre-loxP recombination GEMMs Genotyping Mouse prostate dissection Prostate cancer Syngeneic

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Last Updated: Feb 10, 2026

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Published on: September 18, 2013

Area of Science:

Oncology
Immunology
Genetics

Background:

Cancer research increasingly focuses on the tumor microenvironment.
Immunodeficient models are inadequate for studying immunocompetent anti-tumor responses.
Genetically engineered mouse models (GEMMs) spontaneously develop tumors in an immunocompetent setting.

Purpose of the Study:

To address the limitations of GEMMs in generating synchronized experimental cohorts.
To enable the development of syngeneic models for cancer research.
To facilitate studies on the tumor microenvironment in a fully immunocompetent setting.

Main Methods:

Utilizing Cre-loxP recombination for targeted gene expression in specific tissues.
Deriving cell lines from genetically modified murine prostate tissue.
Generating syngeneic models through subcutaneous or orthotopic injection of derived cell lines.

Main Results:

Established a method for deriving cell lines from GEMMs.
Enabled the generation of age-matched cell lines for synchronized experiments.
Facilitated the creation of syngeneic models for immunocompetent cancer studies.

Conclusions:

Cell line derivation from GEMMs overcomes critical limitations in cancer model development.
This approach supports robust in vivo studies of the tumor microenvironment.
Syngeneic models derived from GEMM cell lines are essential for advancing cancer research.