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Related Concept Videos

¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

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The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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¹H NMR: Pople Notation01:09

¹H NMR: Pople Notation

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The Pople nomenclature system classifies spin systems based on the difference between their chemical shifts. Coupled spins are denoted by capital letters with subscripts indicating the number of equivalent nuclei. When the coupled nuclei have well-separated chemical shifts, they are assigned letters that are far apart in the alphabet, such as A and X. When the difference in chemical shifts is small, coupled nuclei are named using adjacent letters of the alphabet (AB, MN, or XY).
A proton...
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NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

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A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
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Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
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NMR-Based Prostate Cancer Metabolomics.

Leslie R Euceda1, Maria K Andersen1, May-Britt Tessem1

  • 1Department of Circulation and Medical Imaging, NTNU - The Norwegian University of Science and Technology, Trondheim, Norway.

Methods in Molecular Biology (Clifton, N.J.)
|May 23, 2018
PubMed
Summary

This study details Nuclear Magnetic Resonance (NMR) spectroscopy protocols for prostate cancer metabolomics. It provides methods for analyzing cell cultures, biofluids, and tissues to understand metabolic reprogramming in cancer.

Keywords:
BiobankingBiofluids analysisCell extracts analysisMetabolite quantificationMetabolomicsNMR pulse sequencesNMR spectroscopyProstate cancerSample preparationTargeted metabolic pathway analysisTissue analysis

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Area of Science:

  • Oncology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Prostate cancer is a leading cause of cancer death in men globally.
  • Distinguishing indolent from aggressive prostate cancer remains a clinical challenge.
  • Metabolic reprogramming is a key hallmark of cancer, impacting nutrient utilization and energy production.

Purpose of the Study:

  • To provide standardized protocols for Nuclear Magnetic Resonance (NMR) based metabolomics in prostate cancer research.
  • To enable accurate analysis of metabolic alterations in various sample types relevant to prostate cancer.
  • To offer guidance on optimal biobanking and sample preparation for reliable metabolomic profiling.

Main Methods:

  • Detailed protocols for NMR spectroscopy applied to prostate cancer cell cultures.
  • Methods for metabolomic analysis of biofluids, including serum and urine.
  • Procedures for NMR-based metabolomics of intact prostate tissue samples.

Main Results:

  • Established comprehensive NMR metabolomics workflows for prostate cancer research.
  • Demonstrated the utility of NMR spectroscopy in characterizing cancer metabolism.
  • Provided essential guidelines for sample handling to ensure data integrity.

Conclusions:

  • NMR-based metabolomics offers a powerful approach for studying prostate cancer.
  • Standardized protocols are crucial for reproducible and reliable results in cancer metabolomics.
  • This work facilitates deeper understanding of prostate cancer's metabolic landscape to aid clinical differentiation.