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Updated: Feb 10, 2026
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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Published on: December 22, 2014
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Metagenomic study of bacterial microbiota in persistent endodontic infections using Next-generation sequencing
G Sánchez-Sanhueza1, H Bello-Toledo2, G González-Rocha2
1Discipline of Endodontics, Faculty of Dentistry, Department of Restorative Dentistry, University of Concepción, Concepción, Chile.
International Endodontic Journal
|May 23, 2018
Summary
Persistent apical periodontitis involves diverse bacterial communities, particularly Proteobacteria. Clinical factors like American Society of Anesthesiologists (ASA) classification and Periapical Index (PAI) scores correlate with this microbial diversity.
Area of Science:
- Microbiology
- Endodontics
- Genomics
Background:
- Persistent apical periodontitis is a complex oral infection.
- Understanding the root canal microbiota is crucial for effective treatment.
- Next-generation sequencing (NGS) offers advanced insights into microbial communities.
Purpose of the Study:
- To identify bacterial microbiota in persistent apical periodontitis root canals.
- To explore the relationship between microbial communities and patient clinical characteristics.
- To utilize NGS for detailed metagenetic analysis.
Main Methods:
- Bacterial DNA extraction from root canal samples of 24 patients undergoing retreatment.
- Amplification of 16S rRNA gene's V3-V4 regions.
- Deep sequencing using Illumina technology for metagenetic analysis.
Main Results:
- Identified 86 operational taxonomic units (OTUs), with high diversity recovery (99.9%).
- Proteobacteria was the dominant bacterial phylum.
- Higher microbiota richness and distinct phylogenetic relationships were observed in American Society of Anesthesiologists (ASA) II-III patients compared to ASA I.
- Increased microbiota diversity correlated with Periapical Index (PAI) score 5 versus PAI score 4; symptomatic patients showed reduced PAI scores.
Conclusions:
- A strong association exists between clinical features and increased microbiota diversity in persistent endodontic infections.
- This study enhances understanding of host-microbial interactions in endodontic disease.
- Clinical factors may influence or reflect the complexity of the root canal microbiome.
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