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Updated: Feb 10, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Exhaled nitric oxide and mannitol test to predict exercise-induced bronchoconstriction.

Kyubo Kim¹, Hong Je Cho², Jung Won Yoon³

¹Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, Korea.

Pediatrics International : Official Journal of the Japan Pediatric Society

|May 23, 2018

Summary

The fraction of exhaled nitric oxide (FeNO) test and mannitol bronchial provocation test (BPT) can predict exercise-induced bronchoconstriction (EIB) in children with asthma. The FeNO test is a simpler, safer alternative for diagnosing EIB.

Keywords:

asthma exercise-induced bronchoconstriction fractional exhaled nitric oxide mannitol bronchial provocation test

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Area of Science:

Pediatric Pulmonology
Allergy and Immunology
Respiratory Medicine

Background:

Exercise-induced bronchoconstriction (EIB) diagnosis typically requires treadmill exercise challenges, which are resource-intensive.
Alternative diagnostic methods like the fraction of exhaled nitric oxide (FeNO) test and mannitol bronchial provocation test (BPT) are being explored.

Purpose of the Study:

To compare the diagnostic utility of FeNO testing and mannitol BPT in predicting EIB in pediatric asthma patients.
To evaluate FeNO and mannitol BPT as potential surrogates for exercise challenge testing.

Main Methods:

Retrospective analysis of data from 60 asthmatic children (aged 6-16 years).
Comparison of exercise BPT results with FeNO levels and mannitol BPT data.
Correlation analysis and ROC curve analysis to assess diagnostic performance.

Main Results:

Both FeNO levels and mannitol BPT response-dose ratio (RDR) showed significant positive correlations with the severity of EIB.
Receiver operating characteristic (ROC) curve analysis revealed similar areas under the curve (AUC) for FeNO (0.771) and mannitol RDR (0.763) in discriminating EIB.
No significant difference was found between the discriminatory power of FeNO and mannitol RDR.

Conclusions:

FeNO testing and mannitol BPT are both effective in predicting EIB in asthmatic children.
The FeNO test, being simpler and safer, may serve as a clinically valuable standalone diagnostic tool for EIB in this population.