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Short Tandem Repeat (STR) Profiles of Commonly Used Human Ocular Surface Cell Lines
Alison M McDermott1,2, Hasna Baidouri1, Ashley M Woodward3
1a The Ocular Surface Institute , University of Houston College of Optometry , Houston , TX , USA.
Current Eye Research
|May 23, 2018
Summary
This study established short tandem repeat (STR) profiles for human ocular surface cell lines. The verified STR profiles ensure accurate cell line authentication in ocular surface research.
Area of Science:
- Ophthalmology
- Cell Biology
- Genetics
Background:
- Human ocular surface cell lines are crucial for research.
- Cell line authentication is vital for reproducible results.
- Short tandem repeat (STR) profiling is a standard method for cell line identification.
Purpose of the Study:
- To establish and verify the short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
- To provide a reference database for researchers in the field.
Main Methods:
- DNA extraction from multiple passages of human corneal epithelial, conjunctival epithelial, and meibomian gland cell lines.
- Short tandem repeat (STR) analysis performed in an academic setting.
- Comparison of generated STR profiles against available databases.
Main Results:
- Distinct STR profiles were identified for each human corneal epithelial and conjunctival epithelial cell line.
- The meibomian gland cell line exhibited identical STR profiles across samples from different laboratories.
- No cross-contamination with other cell lines, such as HeLa, was detected.
Conclusions:
- The study successfully verified STR profiles for key human ocular surface cell lines.
- These authenticated STR profiles serve as a valuable reference for researchers.
- This resource aids in ensuring the integrity and correct usage of cell lines in ocular surface research.
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