Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

238.3K
Overview
238.3K
Real Time RT-PCR02:57

Real Time RT-PCR

65.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.3K
Quantitative Analysis01:12

Quantitative Analysis

1.5K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.5K
Quantitative Aspects of Drug-Receptor Interaction01:30

Quantitative Aspects of Drug-Receptor Interaction

1.8K
The receptor occupancy theory connects a drug's response to the number of occupied receptors. With higher drug concentrations, more receptors are occupied, leading to increased responses. The formation of drug-receptor complexes involves association and dissociation rates, which reach equilibrium when the forward and backward reactions are equal. The equilibrium association constant (Ka) and its inverse, the equilibrium dissociation constant (Kd), indicate drug affinity. Higher Ka and lower...
1.8K
Reaction Stoichiometry02:57

Reaction Stoichiometry

78.1K
A balanced chemical equation provides a great deal of information in a very succinct format. Chemical formulas provide the identities of the reactants and products involved in the chemical change, allowing classification of the reaction. Coefficients provide the relative numbers of these chemical species, allowing a quantitative assessment of the relationships between the amounts of substances consumed and produced by the reaction. These quantitative relationships are known as the reaction’s...
78.1K
The Scientific Method03:50

The Scientific Method

68.2K
Chemistry is an empirical science. Scientists often pose questions to understand the chemistry in everyday life and seek answers to these questions. To achieve this, scientists follow a definitive series of steps that together make up the Scientific Method. This approach involves making observations, asking questions, building a hypothesis, conducting experiments, analyzing results, and forming a conclusion. 
68.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serodiagnosis of amoebic abscess: a retrospective diagnostic accuracy study of kits marketed in Europe.

Journal of clinical microbiology·2025
Same author

Pragmatic clustered randomised control trial to evaluate a self-regulated learning intervention to be implemented in South Australian primary schools-study protocol.

Trials·2025
Same author

Current knowledge and practice of Candida auris screening in France: A nationwide survey from the French Society of Medical Mycology (SFMM).

Journal de mycologie medicale·2024
Same author

Severe kerion Celsi caused by Trichophyton quinckeanum: Severe kerion Celsi due to Trichophyton quinckeanum.

Journal de mycologie medicale·2023
Same author

Multicentric Analysis of the Species Distribution and Antifungal Susceptibility of Clinical Isolates from Aspergillus Section <i>Circumdati</i>.

Antimicrobial agents and chemotherapy·2023
Same author

Selenium deficiency with high-output heart failure revealed by pigmentary changes ("pseudoalbinism").

Annales de dermatologie et de venereologie·2023

Related Experiment Video

Updated: Feb 10, 2026

Quantitative PCR of T7 Bacteriophage from Biopanning
05:42

Quantitative PCR of T7 Bacteriophage from Biopanning

Published on: September 27, 2018

11.6K

Pneumocystosis and quantitative PCR.

N Issa1, F Gabriel2, G Baulier3

  • 1Réanimation médicale, groupe hospitalier Saint-André, 1, rue Jean-Burguet, 33075 Bordeaux cedex, France; Médecine interne et maladies infectieuses, groupe hospitalier Saint-André, 1, rue Jean-Burguet, 33075 Bordeaux cedex, France.

Medecine Et Maladies Infectieuses
|May 24, 2018
PubMed
Summary
This summary is machine-generated.

Quantitative PCR (qPCR) aids in diagnosing Pneumocystis pneumonia (PCP) in immunosuppressed patients. A qPCR value below 56,000 copies/mL requires clinical correlation for accurate PCP diagnosis.

Keywords:
Pneumocystis pneumoniaPneumonie à pneumocystisRT-PCR

More Related Videos

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
11:44

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

3.0K
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
06:25

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Published on: February 8, 2020

6.1K

Related Experiment Videos

Last Updated: Feb 10, 2026

Quantitative PCR of T7 Bacteriophage from Biopanning
05:42

Quantitative PCR of T7 Bacteriophage from Biopanning

Published on: September 27, 2018

11.6K
Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
11:44

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

3.0K
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
06:25

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Published on: February 8, 2020

6.1K

Area of Science:

  • Medical Diagnostics
  • Infectious Diseases
  • Immunology

Background:

  • Pneumocystis pneumonia (PCP) is increasingly diagnosed in non-HIV immunosuppressed individuals.
  • Traditional direct examination (DE) has limitations in sensitivity for detecting Pneumocystis jirovecii (P. jirovecii).
  • Quantitative PCR (qPCR) offers higher sensitivity for P. jirovecii detection.

Purpose of the Study:

  • To evaluate the diagnostic contribution of quantitative PCR (qPCR) for Pneumocystis pneumonia (PCP).
  • To differentiate between P. jirovecii infection and colonization using qPCR.
  • To assess the utility of qPCR in immunosuppressed, non-HIV patients.

Main Methods:

  • Retrospective study including patients with suspected PCP and positive qPCR.
  • Classification into infection or colonization groups based on clinical, radiological, and microbiological data.
  • Comparison of qPCR values between infected and colonized patients.

Main Results:

  • 150 patients included (75% non-HIV); 129 diagnosed with PCP.
  • Direct examination (DE) was negative in 60% of confirmed PCP cases.
  • Median qPCR: 76,650 copies/mL (infection) vs. 3,220 copies/mL (colonization).
  • 100% specificity threshold at 56,000 copies/mL; optimal differentiation at 10,100 copies/mL.

Conclusions:

  • qPCR is valuable for diagnosing PCP in immunosuppressed patients, particularly when DE is negative.
  • qPCR results <56,000 copies/mL necessitate interpretation alongside clinical and paraclinical findings.
  • qPCR enhances diagnostic accuracy for PCP in at-risk populations.