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Updated: Feb 10, 2026

Ganglioside Extraction, Purification and Profiling
Published on: March 12, 2021
Streamlined chemoenzymatic total synthesis of prioritized ganglioside cancer antigens
Hai Yu1, Abhishek Santra, Yanhong Li
1Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA. xiichen@ucdavis.edu.
Abstract:
A highly efficient streamlined chemoenzymatic strategy for total synthesis of four prioritized ganglioside cancer antigens GD2, GD3, fucosyl GM1, and GM3 from commercially available lactose and phytosphingosine is demonstrated. Lactosyl sphingosine (LacβSph) was chemically synthesized (on a 13 g scale), subjected to sequential one-pot multienzyme (OPME) glycosylation reactions with facile C18-cartridge purification, followed by improved acylation conditions to form target gangliosides, including fucosyl GM1 which has never been synthesized before.
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