Related Experiment Video
Updated: Feb 10, 2026
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
Published on: April 19, 2017
In vitro coagulation triggers anti-Aspergillus fumigatus neutrophil response
Emilie Fréalle1,2, Philippe Gosset1, Sophie Leroy2
1Univ Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection & Immunity of Lille, F-59000 Lille, France.
Aim:
To explore Aspergillus interactions with platelets in the blood, especially during clot formation.
Materials & Methods:
Aspergillus fumigatus resting or swollen conidia, germlings or hyphae were inoculated into blood sampled into tubes with or without anticoagulant. Interactions were explored using microscopy, and chemokine levels were determined.
Results:
Anatomopathological examination of the clot revealed conidia and germlings colocalization with platelet aggregates, and neutrophil recruitment around aggregates. Transmission electron microscopy showed conidia and hyphae surrounded by neutrophils. Increased CCL5 and CXCL4 when conidia or germlings but not hyphae were added suggested they could be involved in neutrophil recruitment around aggregates.
Conclusion:
These data suggest platelets could trigger coagulopathy and activate neutrophils during aspergillosis. They open up new perspectives for aspergillosis management.
Insights
Platelets interact with Aspergillus fungi in blood, potentially triggering blood clots and activating immune cells. This discovery offers new insights for managing invasive aspergillosis infections.
Area of Science:
- Mycology
- Hematology
- Immunology
Background:
- Invasive aspergillosis poses a significant threat, particularly in immunocompromised individuals.
- Understanding fungal-host interactions is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the interactions between Aspergillus fumigatus and human platelets during blood clot formation.
- To elucidate the role of these interactions in the host immune response.
Main Methods:
- Aspergillus fumigatus conidia, germlings, and hyphae were incubated with human blood.
- Microscopy (including transmission electron microscopy) was used to visualize interactions.
- Chemokine levels (CCL5, CXCL4) were measured.
Main Results:
- Aspergillus conidia and germlings localized with platelet aggregates in clots.
- Neutrophil recruitment was observed around these aggregates.
- Increased CCL5 and CXCL4 levels correlated with the presence of conidia/germlings, suggesting a role in neutrophil activation.
Conclusions:
- Platelets may play a role in triggering coagulopathy and activating neutrophils during Aspergillus infections.
- These findings open new therapeutic avenues for managing aspergillosis.
More Related Videos
09:43Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
Published on: December 7, 2019
15:01Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
Published on: September 18, 2021
Related Concept Videos
01:06Coagulation
01:09Coagulation
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
01:36Humoral Immune Responses
02:26Responses to Gravity and Touch
02:00Photoreceptors and Plant Responses to Light
01:22Waterproofing and Anti-Bacterial Admixtures in Concrete
Waterproofing admixtures render concrete hydrophobic,...