Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maturation of Endosomes01:28

Maturation of Endosomes

5.9K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
5.9K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

12.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.9K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

4.9K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.9K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.6K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.6K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

111.1K
Overview
111.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Catestatin restores cardiac metabolic flexibility and enhances mitochondrial function.

Communications biology·2026
Same author

RNA promotes synapsin phase separation providing a platform for local translation.

bioRxiv : the preprint server for biology·2026
Same author

Membrane-protein-mediated phase separation orchestrates organelle contact sites.

Molecular cell·2026
Same author

What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation?

Advanced biology·2025
Same author

Phosphorylation of VAMP3 couples IL-6 exocytosis to dendritic cell activation.

Journal of cell science·2025
Same author

Immunomodulatory effects of 4-hydroxynonenal.

Redox biology·2025

Related Experiment Video

Updated: Feb 10, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.0K

Endosomal and Phagosomal SNAREs.

Ilse Dingjan1, Peter T A Linders1, Danielle R J Verboogen1

  • 1Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center , Nijmegen , The Netherlands ; and Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen , Groningen , The Netherlands.

Physiological Reviews
|May 24, 2018
PubMed
Summary

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins are crucial for organelle communication and intracellular transport. This review highlights SNAREs in endosomal and phagosomal trafficking, noting their roles in pathogen interactions.

More Related Videos

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
10:33

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

11.2K
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

14.4K

Related Experiment Videos

Last Updated: Feb 10, 2026

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

4.0K
Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
10:33

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

11.2K
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
14:39

Measuring Phagosome pH by Ratiometric Fluorescence Microscopy

Published on: December 7, 2015

14.4K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein family mediates essential membrane fusion events for intracellular transport.
  • SNAREs facilitate trafficking between various organelles, including endosomes, phagosomes, lysosomes, Golgi, plasma membrane, and endoplasmic reticulum.

Purpose of the Study:

  • To provide a comprehensive overview of SNARE proteins involved in endosomal and phagosomal trafficking pathways.
  • To explore the significant roles of SNAREs in the context of pathogen manipulation of host cell transport.

Main Methods:

  • Literature review focusing on SNARE protein functions in endocytic and phagocytic pathways.
  • Analysis of identified SNAREs in human endosomes and phagosomes.

Main Results:

  • Out of 38 human SNAREs, 30 are localized to endosomes and/or phagosomes.
  • Many SNAREs are targeted by viruses and intracellular pathogens to hijack cellular transport.
  • Pathogen exploitation of SNAREs aids nutrient acquisition, immune evasion, and prevents degradation.

Conclusions:

  • SNARE proteins are central players in the complex network of endosomal and phagosomal trafficking.
  • Understanding SNARE function is critical for deciphering pathogen-host interactions and developing therapeutic strategies.