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Updated: Feb 10, 2026
12:23
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
14.9K
Fluorescence resonance energy-transfer-based fluoride ion sensor
Applied Optics
|May 24, 2018
Summary
This study presents a new sensor for detecting fluoride using a dye pair. The sensor shows high specificity for fluoride detection by monitoring fluorescence changes.
Area of Science:
- Analytical Chemistry
- Photochemistry
- Materials Science
Background:
- Fluorescence resonance energy transfer (FRET) is a powerful tool for molecular sensing.
- Developing highly specific and sensitive sensors for anions like fluoride remains a challenge.
- Coumarin 540a (C540a) and Rhodamine 6g (Rh6g) are photostable dyes suitable for FRET applications.
Purpose of the Study:
- To develop and characterize an energy-transfer-based sensor for fluoride detection.
- To investigate the sensing mechanism and specificity of the C540a-Rh6g dye pair towards fluoride.
- To establish a broad-range fluoride detection method using fluorescence monitoring.
Main Methods:
- Utilized a Coumarin 540a (donor) and Rhodamine 6g (acceptor) dye pair for energy transfer studies.
- Investigated the effect of varying fluoride concentrations on the fluorescence emission of both dyes in acetonitrile.
- Analyzed the fluorescence resonance energy transfer efficiency in response to fluoride and other anions.
Main Results:
- Fluoride concentration was found to decrease the energy transfer efficiency between C540a and Rh6g.
- A recovery of fluorescence emission from C540a was observed with increasing fluoride levels.
- The sensor demonstrated high specificity for fluoride compared to other tested anions.
- Broad-range fluoride detection was achieved by monitoring the fluorescence of both dyes.
Conclusions:
- The C540a-Rh6g dye pair enables effective energy-transfer-based fluoride sensing.
- The developed sensor exhibits high specificity and a broad detection range for fluoride.
- This approach offers a promising method for sensitive and selective fluoride detection.
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